Clinical Tools and Equipment

Each Kit Contains:

• Artificial Seminiferous Tubule (0.3 micronx10 cm)
• Loading Media                                                                
• Maturation Media                                                         
• Collection Media
• 5 cm Luer lock syringes 

Keep the kit at 4C

Applications: Visualization and microscopic evaluation of spermatogenesis. Note, the kit has only been validated for mouse.

Expiration Dates:

Tubule: 3 months

Media:  1 month

                  Preparation of Artificial Seminiferous Tubules:

• In a bio safety cabinet open the sleeve of the Artificial Tubule
• Using a sterile tweezers transfer the tubule into a sterile 6 cm dish
• Connect a 5 cm syringe to the port of the Tubule
• Load 10-20 micro liters of 2-5 days old mouse testicular cells (50-100 millions/ml) util the tubule is filled 
• Place the tubule in the 6cm dish
• All 10 ml of loading media
• Place the dish in a 32C incubator with 5% CO2
• Perform complete media change at day 3 and 5
• At day 7, remove all the loading media and all 10 ml of maturation media
• Perform complete media change at day 10 and 12
• At day 14, using the other 5 ml syringe and collection media flush the cells out of the artificial seminiferous tubule
• At this time about 80% of the cells should be haploid mainly round spermatids
• Extending maturation to 21 days may result in limited number of more advanced spermatogenic cells such as elongated spermatids.