Clinical Tools and Equipment
Bone Marrow
Medical Supplies and Equipment
Medical Supplies and Equipment
- Bone marrow aspiration needles
- Bone marrow separation kits medical tools
Medical Supplies and Equipment
Medical Supplies and Equipment
Medical Supplies and Equipment
- PRP separation kits
- PRP machines centrifuge
Adipose Tissue
Medical Supplies and Equipment
Adipose Tissue
- Adipose tissue dissociation kits
- SVF isolation machines centrifuge
Diagnostic Tools
Immunofluorescense Analyzer
The C-reactive protein (CRP) is synthesized by the liver in response to interleukin-6 and is well known as one of the classical acute-phase reacants. It is used as a marker of inflammation and cardiovascular diseases
Research Tools
Spermatogenic Maturation Kit "For Research Only"
Spermatogenic Maturation Kit "For Research Only"
Spermatogenic Maturation Kit "For Research Only"
Each Kit Contains:
- Artificial Seminiferous Tubule (0.3 micronx10 cm)
- Loading Media
- Maturation Media
- Collection Media
- 5 cm Luer lock syringes
Keep the kit at 4C
Applications: Visualization and microscopic evaluation of spermatogenesis. Note, the kit has only been validated for mouse.
Expiration Dates:
Tubule: 3 months
Media: 1 month
Instructions
Spermatogenic Maturation Kit "For Research Only"
Spermatogenic Maturation Kit "For Research Only"
Preparation of Artificial Seminiferous Tubules:
- In a bio safety cabinet open the sleeve of the Artificial Tubule
- Using a sterile tweezers transfer the tubule into a sterile 6 cm dish
- Connect a 5 cm syringe to the port of the Tubule
- Load 10-20 micro liters of 2-5 days old mouse testicular cells (50-100 millions/ml) util the tubule is filled
- Place the tubule in the 6cm dish
- All 10 ml of loading media
- Place the dish in a 32C incubator with 5% CO2
- Perform complete media change at day 3 and 5
- At day 7, remove all the loading media and all 10 ml of maturation media
- Perform complete media change at day 10 and 12
- At day 14, using the other 5 ml syringe and collection media flush the cells out of the artificial seminiferous tubule
- At this time about 80% of the cells should be haploid mainly round spermatids
- Extending maturation to 21 days may result in limited number of more advanced spermatogenic cells such as elongated spermatids.
